RNA isolation / purification Yeast - Ashbya gossypii

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.

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Found 2 matching solutions for this experiment

RiboPure™ RNA Purification Kit, yeast

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
Samples can be flash frozen (-80C) before use, this will likely cause lower RNA yield.		When checking the yield by means of a spectrophotometer, use the supplied TE buffer to zero the machine

Upstream tips
Samples can be flash frozen (-80C) before use, this will likely cause lower RNA yield.
Downstream tips
When checking the yield by means of a spectrophotometer, use the supplied TE buffer to zero the machine

Manufacturer protocol Publication protocol 1 Relevant paper

RNeasy Plant Mini Kit

Qiagen

Upstream tips	Protocol tips	Downstream tips
	Both enzymatic and mechanical lysis can be used for yeast. Enzymatic lysis doesn't require additional lab equipment, but takes longer than mechanical disruption.

Protocol tips
Both enzymatic and mechanical lysis can be used for yeast. Enzymatic lysis doesn't require additional lab equipment, but takes longer than mechanical disruption.

Manufacturer protocol Publication protocol 1 Relevant paper

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