No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
|
The main characteristics of the different assays were as follows: (1) the laboratory associated with the NRC in-house consensus HDV real-time RT-PCR method (NRC-hepatitis delta virus quantification [HDVQ]), (2) the Roche Lightmix HDV kit (Lightmix Roche), (3) the Robogene HDV RNA quantification kit (Aj-Roboscreen), and (4) the DiaPro HDV RNA Quantification (QT) (DiaPro) are summarized in Table 1. |
|
Protocol tips |
The main characteristics of the different assays were as follows: (1) the laboratory associated with the NRC in-house consensus HDV real-time RT-PCR method (NRC-hepatitis delta virus quantification [HDVQ]), (2) the Roche Lightmix HDV kit (Lightmix Roche), (3) the Robogene HDV RNA quantification kit (Aj-Roboscreen), and (4) the DiaPro HDV RNA Quantification (QT) (DiaPro) are summarized in Table 1. |
Upstream tips |
Protocol tips |
Downstream tips |
|
Prepare standard 20 uL final reaction mix.
Reverse transcribe at 45°C for 10 min, initiate polymerase activation at 95°C for 2min |
If PCR efficiency below 90% Increase extension time/Increase concentration of primers in 100nM increments |
Protocol tips |
Prepare standard 20 uL final reaction mix.
Reverse transcribe at 45°C for 10 min, initiate polymerase activation at 95°C for 2min |
Downstream tips |
If PCR efficiency below 90% Increase extension time/Increase concentration of primers in 100nM increments |
Upstream tips |
Protocol tips |
Downstream tips |
After reverse transcription,start the PCR with an initial incubation step of 5 min at 95°C to activate HotStarTaq Plus DNA Polymerase. |
Program the real-time cycler according to the product protocol |
|
Upstream tips |
After reverse transcription,start the PCR with an initial incubation step of 5 min at 95°C to activate HotStarTaq Plus DNA Polymerase. |
Protocol tips |
Program the real-time cycler according to the product protocol |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!