RNA sequencing Human - Huh7

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Start discussion

No discussions found

Share your thoughts or question with experts in your field

Start a discussion

Found 2 matching solutions for this experiment

Ion Total RNA-Seq Kit v2

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	To reduce fragmentation variability, accurately pipet 1 μL of 10X RNase III Reaction Buffer and 1 μL of RNase III to each sample. Do not make a master mix that contains only 10X RNase III Reaction Buffer and RNase III.

Protocol tips
To reduce fragmentation variability, accurately pipet 1 μL of 10X RNase III Reaction Buffer and 1 μL of RNase III to each sample. Do not make a master mix that contains only 10X RNase III Reaction Buffer and RNase III.

Manufacturer protocol Publication protocol 1 Relevant paper

TruSeq RNA Library Prep Kit v2

Illumina

Upstream tips	Protocol tips	Downstream tips
	0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Protocol tips
0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Fill out your contact details and receive price quotes in your Inbox