RNA sequencing Rat - INS-1 832/13

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

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TruSeq RNA Library Prep Kit v2

Illumina

Upstream tips	Protocol tips	Downstream tips
	Each library was generated using Illumina's “TruSeq RNA Sample Preparation v2 Guide” (Illumina Part no. 15026495, Rev. C) and Illumina's TruSeq RNA Sample Prep Kit v2 (Illumina Inc., San Diego, CA). mRNA was purified from 1 μg of total RNA using poly-T oligo-attached magnetic beads.

Protocol tips
Each library was generated using Illumina's “TruSeq RNA Sample Preparation v2 Guide” (Illumina Part no. 15026495, Rev. C) and Illumina's TruSeq RNA Sample Prep Kit v2 (Illumina Inc., San Diego, CA). mRNA was purified from 1 μg of total RNA using poly-T oligo-attached magnetic beads.

Manufacturer protocol Publication protocol 1 Relevant paper

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