ROS assay cell type - 3T3-L1

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Upstream tips
- Cells were pretreated with NAC (10 μM) for 1h.
Protocol tips
- Cells after pretreating with NAC are treated with 5 μM
CM-H2DCFDA or 10 μM DHE for 30 min at 37 °C.

- Tissues were treated with 5 μM CM-H2DCFDA or 10 μM DHE at room temperature for 30 min
Protocol tips
- Cells were treated with Oxidative Stress Detection Reagent after treating them with fixatives (4% paraformaldehyde and 100% acetone).

- Cells were also immunostained with anti-perilipin antibody and TRITC-conjugated anti-rabbit IgG antibody.
Protocol tips
- Differentiated 3T3-L1 cells were cultured for another 2 days and assayed using ROS kit.
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