ROS assay cell type - A549 human adenocarcinomic human alveolar basal epithelial cells

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 2 matching solutions for this experiment

Upstream tips
- 100,000 cells per well were seeded in a 12 well plate
Protocol tips
- Cells were treated with 500 μl of ROS/Superoxide Detection Mix for 30 minutes at 37°C
Upstream tips
- Cells were pretreated with LGH00168 for 1 h.
Protocol tips
- After washing step, cells were incubated with 5 μmol/L of MitoSOX for 20 min in the dark.
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