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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
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- Cells with treated with oxidative stress detection reagent and superoxide detection reagent for 30 minutes before CNT solution.
- After 60 minutes of Pyocyanin treatement, cells were washed, harvested and treated with trypsinethylenediaminetetraacetic acid.
- Cells were resuspended in 0.3 mL 1× wash buffer with 10% FBS and passed through a nylon mesh; then assessed by FACS. |
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| Protocol tips |
- Cells with treated with oxidative stress detection reagent and superoxide detection reagent for 30 minutes before CNT solution.
- After 60 minutes of Pyocyanin treatement, cells were washed, harvested and treated with trypsinethylenediaminetetraacetic acid.
- Cells were resuspended in 0.3 mL 1× wash buffer with 10% FBS and passed through a nylon mesh; then assessed by FACS. |
| Upstream tips |
Protocol tips |
Downstream tips |
| - Cells were plated at a density of 100,000 cells/well |
- plates were placed in hyperoxia or normoxia for 0 h, 4 h, 8 h, 12 h and 24 h. H2O2 substrate solution was added to the plates 6 h.
- The detection solution was added to the plates 20 min before each read. |
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| Upstream tips |
| - Cells were plated at a density of 100,000 cells/well |
| Protocol tips |
- plates were placed in hyperoxia or normoxia for 0 h, 4 h, 8 h, 12 h and 24 h. H2O2 substrate solution was added to the plates 6 h.
- The detection solution was added to the plates 20 min before each read. |
| Upstream tips |
Protocol tips |
Downstream tips |
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- 1X DCFH-DA/media solution contains 5% methanol. For cells that are sensitive to methanol,
we recommend instead preparing a 0.1X (100 µM) solution of DCFH-DA in cell culture media. |
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| Protocol tips |
- 1X DCFH-DA/media solution contains 5% methanol. For cells that are sensitive to methanol,
we recommend instead preparing a 0.1X (100 µM) solution of DCFH-DA in cell culture media. |
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