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|- Cells with treated with oxidative stress detection reagent and superoxide detection reagent for 30 minutes before CNT solution.
- After 60 minutes of Pyocyanin treatement, cells were washed, harvested and treated with trypsinethylenediaminetetraacetic acid.
- Cells were resuspended in 0.3 mL 1× wash buffer with 10% FBS and passed through a nylon mesh; then assessed by FACS.
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