ROS assay cell type - BEAS-2B human bronchial epithelial cell line

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Protocol tips
- Cells with treated with oxidative stress detection reagent and superoxide detection reagent for 30 minutes before CNT solution.

- After 60 minutes of Pyocyanin treatement, cells were washed, harvested and treated with trypsinethylenediaminetetraacetic acid.

- Cells were resuspended in 0.3 mL 1× wash buffer with 10% FBS and passed through a nylon mesh; then assessed by FACS.
Upstream tips
- Cells were plated at a density of 100,000 cells/well
Protocol tips
- plates were placed in hyperoxia or normoxia for 0 h, 4 h, 8 h, 12 h and 24 h. H2O2 substrate solution was added to the plates 6 h.

- The detection solution was added to the plates 20 min before each read.
Protocol tips
- 1X DCFH-DA/media solution contains 5% methanol. For cells that are sensitive to methanol,
we recommend instead preparing a 0.1X (100 µM) solution of DCFH-DA in cell culture media.
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