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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - 25000 cells per well were platted in 96 well plates in DMEM without phenol red |
- After 24h cells were treated with 25 μM DCFDA and incubated for 45 min at 37°C.
- For 10 min to 1 h they were treated with 0, 500 or 1000 μM H2O2. |
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| Upstream tips |
| - 25000 cells per well were platted in 96 well plates in DMEM without phenol red |
| Protocol tips |
- After 24h cells were treated with 25 μM DCFDA and incubated for 45 min at 37°C.
- For 10 min to 1 h they were treated with 0, 500 or 1000 μM H2O2. |
| Upstream tips |
Protocol tips |
Downstream tips |
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- Wells were loaded with Amplex red and exposed to CTX.
- For detection of cellular oxidants, cells loaded with dihydroethidium were exposed to CTX for 1 h and assayed. |
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| Protocol tips |
- Wells were loaded with Amplex red and exposed to CTX.
- For detection of cellular oxidants, cells loaded with dihydroethidium were exposed to CTX for 1 h and assayed. |
| Upstream tips |
Protocol tips |
Downstream tips |
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- Cells were incubated with 1 mM H2O2 for 30 min prior to lysis. |
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| Protocol tips |
| - Cells were incubated with 1 mM H2O2 for 30 min prior to lysis. |
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