ROS assay cell type - CHO-K1

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 2 matching solutions for this experiment

CM-H2DCFDA (General Oxidative Stress Indicator)

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
- 2.5 × 104 cells were plated in 24-well plate. - After 24h incubation, cells were pre-treated with SPIONs for 48 h at a final concentration of 200 µg Fe/mL	- After washing, cells were ytreated with DCFDA in 1xPBS at a concentration of 7 µM at at 37 °C for 45 min.

Upstream tips
- 2.5 × 104 cells were plated in 24-well plate. - After 24h incubation, cells were pre-treated with SPIONs for 48 h at a final concentration of 200 µg Fe/mL
Protocol tips
- After washing, cells were ytreated with DCFDA in 1xPBS at a concentration of 7 µM at at 37 °C for 45 min.

Manufacturer protocol Publication protocol 1 Relevant paper

Diogenes

National Diagnostics

Upstream tips	Protocol tips	Downstream tips
- 75,000 cells/well were platted for the assay	- After 48h of post transfection, cells were for ROS with or without activation with 2 μg/ml phorbol myristate acetate

Upstream tips
- 75,000 cells/well were platted for the assay
Protocol tips
- After 48h of post transfection, cells were for ROS with or without activation with 2 μg/ml phorbol myristate acetate

Manufacturer protocol Publication protocol 1 Relevant paper

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