ROS assay cell type - CHO-K1

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 2 matching solutions for this experiment

Upstream tips
- 2.5 × 104 cells were plated in 24-well plate.

- After 24h incubation, cells were pre-treated with SPIONs for 48 h at a final concentration of 200 µg Fe/mL
Protocol tips
- After washing, cells were ytreated with DCFDA in 1xPBS at a concentration of 7 µM at at 37 °C for 45 min.

National Diagnostics

Upstream tips
- 75,000 cells/well were platted for the assay
Protocol tips
- After 48h of post transfection, cells were for ROS with or without activation with 2 μg/ml phorbol myristate acetate
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms