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Found 2 matching solutions for this experiment
|- Cells were pretreated with SiNPs at different concentrations (0, 25, 50, 100 μg/ml) for 24 h and 48 h.
|- Hydrogen peroxide 20 μM concentration was used for the assay.
- After adding DCFH, plate was incubated for 30min.
|- Cells were treated with 25 μM DCFDA in essential medium with 10% FBS in 37°C incubator for 45 min.
- Before analysing cells, they are treated with hyperosmotic media by adding NaCl for different time periods (30–180 min).
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