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Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
- 5 × 105 per well were seeded. |
- Cells were treated at 37 °C for 60 min with DCF dye.
- Cells were treated and incubated with 12AC2O, 3O, 4O, 5O or 6O, each at 10 µM, in a 12-well plate at 37 °C for 8 h |
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Upstream tips |
- 5 × 105 per well were seeded. |
Protocol tips |
- Cells were treated at 37 °C for 60 min with DCF dye.
- Cells were treated and incubated with 12AC2O, 3O, 4O, 5O or 6O, each at 10 µM, in a 12-well plate at 37 °C for 8 h |
Upstream tips |
Protocol tips |
Downstream tips |
- 1.5×105 cells/well in 96-well plates.
- Cells were treated with 20 µM BL or liposomal BL for 48 h. |
- 20 µM of DCFDA was added to cells and incubated for 37°C for further 30 min in the dark. |
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Upstream tips |
- 1.5×105 cells/well in 96-well plates.
- Cells were treated with 20 µM BL or liposomal BL for 48 h. |
Protocol tips |
- 20 µM of DCFDA was added to cells and incubated for 37°C for further 30 min in the dark. |
Upstream tips |
Protocol tips |
Downstream tips |
- 1 × 10 ^5 cells were seeded. |
-positive control cells were incubated for 30 min at 37 °C in dark |
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Upstream tips |
- 1 × 10 ^5 cells were seeded. |
Protocol tips |
-positive control cells were incubated for 30 min at 37 °C in dark |
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