ROS assay cell type - K562 human leukemia cells

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Upstream tips
- 5 × 105 per well were seeded.
Protocol tips
- Cells were treated at 37 °C for 60 min with DCF dye.

- Cells were treated and incubated with 12AC2O, 3O, 4O, 5O or 6O, each at 10 µM, in a 12-well plate at 37 °C for 8 h
Upstream tips
- 1.5×105 cells/well in 96-well plates.

- Cells were treated with 20 µM BL or liposomal BL for 48 h.
Protocol tips
- 20 µM of DCFDA was added to cells and incubated for 37°C for further 30 min in the dark.
Upstream tips
- 1 × 10 ^5 cells were seeded.
Protocol tips
-positive control cells were incubated for 30 min at 37 °C in dark
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