ROS assay cell type - L-02 human fetal hepatocyte

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 2 matching solutions for this experiment

DCFDA / H2DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit

Abcam

Upstream tips	Protocol tips	Downstream tips
	- Cells were treated with 10 μM DCFDA and incubated for 30 min at 37°C in the dark

Protocol tips
- Cells were treated with 10 μM DCFDA and incubated for 30 min at 37°C in the dark

Manufacturer protocol Publication protocol 1 Relevant paper

DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit

Abcam

Upstream tips	Protocol tips	Downstream tips
	- After adding DCFDA 10 μM, cells were incubated for 30 min at 37°C in the dark

Protocol tips
- After adding DCFDA 10 μM, cells were incubated for 30 min at 37°C in the dark

Manufacturer protocol Publication protocol 1 Relevant paper

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