ROS assay cell type - MCF-7

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 2 matching solutions for this experiment

ROS-ID® Total ROS/Superoxide detection kit

Enzo Life Sciences

Upstream tips	Protocol tips	Downstream tips
-Cells should be healthy and not overcrowded since results of the experiments will depend significantly on the cells’ condition.	- Cells should be pre-treated with the ROS Inhibitor at least 30 minutes prior to induction.

Upstream tips
-Cells should be healthy and not overcrowded since results of the experiments will depend significantly on the cells’ condition.
Protocol tips
- Cells should be pre-treated with the ROS Inhibitor at least 30 minutes prior to induction.

Manufacturer protocol Publication protocol 1 Relevant paper

Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
- cells were grown on glass cover slips placed in 12-well plate	- Cells were treated with 12.5 µM and 25 µM concentration of HHQ for 48 h

Upstream tips
- cells were grown on glass cover slips placed in 12-well plate
Protocol tips
- Cells were treated with 12.5 µM and 25 µM concentration of HHQ for 48 h

Manufacturer protocol Publication protocol 1 Relevant paper

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