ROS assay cell type - MCF-7

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Upstream tips
-Cells should be healthy and not overcrowded since results of the experiments will depend significantly on the cells’ condition.
Protocol tips
- Cells should be pre-treated with the ROS Inhibitor at least 30 minutes prior to induction.
Upstream tips
- cells were grown on glass cover slips placed in 12-well plate
Protocol tips
- Cells were treated with 12.5 µM and 25 µM concentration of HHQ for 48 h
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