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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - 10,000 MDA-MB-231 cells per well in a total volume of 70 µl. |
- After adding the test compunds and H2O2 solution, the plate was incubated at 37°C in a 5% CO2 incubator for 60 min.
- After adding ROS-Glo detection solution, plate was incubated for an additional 20 min |
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| Upstream tips |
| - 10,000 MDA-MB-231 cells per well in a total volume of 70 µl. |
| Protocol tips |
- After adding the test compunds and H2O2 solution, the plate was incubated at 37°C in a 5% CO2 incubator for 60 min.
- After adding ROS-Glo detection solution, plate was incubated for an additional 20 min |
| Upstream tips |
Protocol tips |
Downstream tips |
| - Cells were pre treated with 8.45 μm JAHA |
- After 24 and 48 h of incubation and resuspended in 500 μL of ROS/ Superoxide Detection Mix and incubated for 30 min at 37 °C in the dark |
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| Upstream tips |
| - Cells were pre treated with 8.45 μm JAHA |
| Protocol tips |
| - After 24 and 48 h of incubation and resuspended in 500 μL of ROS/ Superoxide Detection Mix and incubated for 30 min at 37 °C in the dark |
| Upstream tips |
Protocol tips |
Downstream tips |
- 10,000 cells were seeded in 96-well.
- Cells were incubated with serum free medium fro 24h before treated with DCFDA. |
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| Upstream tips |
- 10,000 cells were seeded in 96-well.
- Cells were incubated with serum free medium fro 24h before treated with DCFDA. |
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