ROS assay cell type - MDA-MB-231

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Upstream tips
- 10,000 MDA-MB-231 cells per well in a total volume of 70 µl.
Protocol tips
- After adding the test compunds and H2O2 solution, the plate was incubated at 37°C in a 5% CO2 incubator for 60 min.

- After adding ROS-Glo detection solution, plate was incubated for an additional 20 min
Upstream tips
- Cells were pre treated with 8.45 μm JAHA
Protocol tips
- After 24 and 48 h of incubation and resuspended in 500 μL of ROS/ Superoxide Detection Mix and incubated for 30 min at 37 °C in the dark
Upstream tips
- 10,000 cells were seeded in 96-well.

- Cells were incubated with serum free medium fro 24h before treated with DCFDA.
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