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Found 4 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
- cells were pre-treated with or without NAC (30 mM) for
1 h,
-cells were further incubated in the absence or the presence
of gemcitabine (10 mM) for 24 h |
- with ROS/superoxide detection solution for 10 min at
37 C |
|
| Upstream tips |
- cells were pre-treated with or without NAC (30 mM) for
1 h,
-cells were further incubated in the absence or the presence
of gemcitabine (10 mM) for 24 h |
| Protocol tips |
- with ROS/superoxide detection solution for 10 min at
37 C |
| Upstream tips |
Protocol tips |
Downstream tips |
|
The generation of other ROS by BITC treatment was measured using hROS detection kit (Cell Technology) according to user's manual with slight modification. |
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| Protocol tips |
| The generation of other ROS by BITC treatment was measured using hROS detection kit (Cell Technology) according to user's manual with slight modification. |
| Upstream tips |
Protocol tips |
Downstream tips |
| - 1X104 cells were seeded and incubate overnight at 37C. |
- Cells were treated with DCFH-DA in 100 μL/well for 45 min at 37°C. |
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| Upstream tips |
| - 1X104 cells were seeded and incubate overnight at 37C. |
| Protocol tips |
| - Cells were treated with DCFH-DA in 100 μL/well for 45 min at 37°C. |
| Upstream tips |
Protocol tips |
Downstream tips |
| - It is recommended to keep the final concentration of solvents such as DMSO to ≤1%. |
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| Upstream tips |
| - It is recommended to keep the final concentration of solvents such as DMSO to ≤1%. |
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