ROS assay cell type - MiaPaCa-2 pancreatic carcinoma

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 4 matching solutions for this experiment

Upstream tips
- cells were pre-treated with or without NAC (30 mM) for
1 h,

-cells were further incubated in the absence or the presence
of gemcitabine (10 mM) for 24 h
Protocol tips
- with ROS/superoxide detection solution for 10 min at
37 C
Fluoro hROS

Cell Technology

Protocol tips
The generation of other ROS by BITC treatment was measured using hROS detection kit (Cell Technology) according to user's manual with slight modification.
Upstream tips
- 1X104 cells were seeded and incubate overnight at 37C.
Protocol tips
- Cells were treated with DCFH-DA in 100 μL/well for 45 min at 37°C.
Upstream tips
- It is recommended to keep the final concentration of solvents such as DMSO to ≤1%.
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