ROS assay cell type - mouse splenocytes

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Start discussion

No discussions found

Share your thoughts or question with experts in your field

Start a discussion

Found 2 matching solutions for this experiment

CellROX™ Deep Red Reagent, for oxidative stress detection

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
- 10^6 cells were seeded.	- Cells were treated with 5 µM CellROX Deep Red or 10 µM MitoPY1 in HBSS containing 2.5 mM of probenecid.

Upstream tips
- 10^6 cells were seeded.
Protocol tips
- Cells were treated with 5 µM CellROX Deep Red or 10 µM MitoPY1 in HBSS containing 2.5 mM of probenecid.

Manufacturer protocol Publication protocol 1 Relevant paper

DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit

Abcam

Upstream tips	Protocol tips	Downstream tips
- 1.5 × 10^5 cells were platted	- 20 μM DCFDA was treated to the cells

Upstream tips
- 1.5 × 10^5 cells were platted
Protocol tips
- 20 μM DCFDA was treated to the cells

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Fill out your contact details and receive price quotes in your Inbox