ROS assay cell type - mouse splenocytes

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 2 matching solutions for this experiment

Upstream tips
- 10^6 cells were seeded.
Protocol tips
- Cells were treated with 5 µM CellROX Deep Red or 10 µM MitoPY1 in HBSS containing 2.5 mM of probenecid.
Upstream tips
- 1.5 × 10^5 cells were platted
Protocol tips
- 20 μM DCFDA was treated to the cells
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