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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
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- After cells were treated with soluble RANKL for 6h, they are washed and cultured in a medium with 2% FBS.
Cells were treated with oxidative stress detection reagent for 30 min on ice |
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| Protocol tips |
- After cells were treated with soluble RANKL for 6h, they are washed and cultured in a medium with 2% FBS.
Cells were treated with oxidative stress detection reagent for 30 min on ice |
| Upstream tips |
Protocol tips |
Downstream tips |
| - 1x10^4 cells were seeded for an assay. |
- After 48h of culture, cells were treated with ROS/Superoxide Detection Mix for 30 min at 37°C |
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| Upstream tips |
| - 1x10^4 cells were seeded for an assay. |
| Protocol tips |
| - After 48h of culture, cells were treated with ROS/Superoxide Detection Mix for 30 min at 37°C |
| Upstream tips |
Protocol tips |
Downstream tips |
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- Before treating cells with DCFH-DA treatment for 60 min at 37°C cells were washed with prechilled PBS. |
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| Protocol tips |
| - Before treating cells with DCFH-DA treatment for 60 min at 37°C cells were washed with prechilled PBS. |
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