ROS assay cell type - Raw 264.7

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Protocol tips
- After cells were treated with soluble RANKL for 6h, they are washed and cultured in a medium with 2% FBS.

Cells were treated with oxidative stress detection reagent for 30 min on ice
Upstream tips
- 1x10^4 cells were seeded for an assay.
Protocol tips
- After 48h of culture, cells were treated with ROS/Superoxide Detection Mix for 30 min at 37°C
Protocol tips
- Before treating cells with DCFH-DA treatment for 60 min at 37°C cells were washed with prechilled PBS.
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