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Found 1 matching solution for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
|
siRNA concentration- 20 nmol/L
siRNA SEQUENCE- GGAGAUUGAUGGUCUACUA-3′ and 5′-AACAGCCAUGGAUACACUUGATT-3′
Add INTERFERin® to siRNA duplexes and incubate for 10min at RT after homogenization.
Remove culture medium from cells and add incubate mixture.
Incubate the plate at 37°C for 24 to 72 h. |
|
| Protocol tips |
siRNA concentration- 20 nmol/L
siRNA SEQUENCE- GGAGAUUGAUGGUCUACUA-3′ and 5′-AACAGCCAUGGAUACACUUGATT-3′
Add INTERFERin® to siRNA duplexes and incubate for 10min at RT after homogenization.
Remove culture medium from cells and add incubate mixture.
Incubate the plate at 37°C for 24 to 72 h. |
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