siRNA / miRNA gene silencing Human - A172 HES6

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 2 matching solutions for this experiment

Hs_HES6_3 FlexiTube siRNA + FuGENE® 6 Transfection Reagent

Protocol tips
Hs_HES6_3:AAGCTTGAACTTGCCACTTCA

Final concentration of 15 nM.

Incubate the FuGENE 6 Transfection Reagent/medium mixture for 5 minutes at room temperature.

Incubate transfection mixture for 15 min at room temperature and add to cells and shake for 10-30 seconds.

Incubate cells for 24-48 h.

Hs_HES6_1 FlexiTube siRNA. + FuGENE® 6 Transfection Reagent

Protocol tips
Hs_HES6_1:TTGGAGTTAGTTACCCTTGAA

Final concentration of 15 nM.

Incubate the FuGENE 6 Transfection Reagent/medium mixture for 5 minutes at room temperature
Incubate transfection mixture for 15 min at room temperature and add to cells and shake for 10-30 seconds.

Incubate cells for 24-48 h.
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