siRNA / miRNA gene silencing Human - A172 TET1

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

ON-TARGETplus Human TET1 siRNA

Dharmacon

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
Seed 1 × 10^5 cells per well.	Incubate transfection mixture for 10-15 min at room temperature. Replace medium after five hours and incubate for additional 48 h in in 0.2% hypoxic conditions.

Upstream tips
Seed 1 × 10^5 cells per well.
Protocol tips
Incubate transfection mixture for 10-15 min at room temperature. Replace medium after five hours and incubate for additional 48 h in in 0.2% hypoxic conditions.

Manufacturer protocol Publication protocol 1 Relevant paper

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