siRNA / miRNA gene silencing Human - A253 IGFBP-7

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Dharmacon

Takara Bio Inc

Upstream tips	Protocol tips	Downstream tips
Seed 5 × 10^5 cells	Final concentration of siRNAs-30 nM. Add siRNA with Xfect Reaction Buffer and vortex for 10 sec at high speed. Incubate for 10 min at room temperature and add to cells. Incubate cells at 37°C for 4 hr to overnight. Replace medium after 4h and incubate for another 48h.

Upstream tips
Seed 5 × 10^5 cells
Protocol tips
Final concentration of siRNAs-30 nM. Add siRNA with Xfect Reaction Buffer and vortex for 10 sec at high speed. Incubate for 10 min at room temperature and add to cells. Incubate cells at 37°C for 4 hr to overnight. Replace medium after 4h and incubate for another 48h.

Manufacturer protocol Publication protocol 1 Relevant paper

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