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Takara Bio Inc
|Seed 5 × 10^5 cells|
|Final concentration of siRNAs-30 nM.
Add siRNA with Xfect Reaction Buffer and vortex for 10 sec at high speed.
Incubate for 10 min at room temperature and add to cells.
Incubate cells at 37°C for 4 hr to overnight.
Replace medium after 4h and incubate for another 48h.