siRNA / miRNA gene silencing Human - A431 EGFR

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

siRNA EGFR + Lipofectamine® 2000 Transfection Reagent

siRNA EGFR

Thermo Fisher Scientific

Upstream tips
1 × 10^5/well in six-well plates
Protocol tips
EGFR siRNA #2 (ID s564), sense 5′-CCAUAAAUGCUACGAAUAUtt-3′ and antisense 5′-AUAUUCGUAGCAUUUAUGGag-3

Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature.

Incubate cells for 1–3 days at 37°C.
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