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| siRNA concentration-100 nM.
Incubate TransIT-siQUEST Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells.
Incubate for 24–72 hours.
It is not necessary to replace the complete growth medium with fresh medium.
After 48h inoculate with RSV.
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