siRNA / miRNA gene silencing Human - A549 IRF3

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Dharmacon

TransIT-siQUEST

Mirus

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-100 nM. Incubate TransIT-siQUEST Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells. Incubate for 24–72 hours. It is not necessary to replace the complete growth medium with fresh medium. After 48h inoculate with RSV.

Protocol tips
siRNA concentration-100 nM. Incubate TransIT-siQUEST Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells. Incubate for 24–72 hours. It is not necessary to replace the complete growth medium with fresh medium. After 48h inoculate with RSV.

Manufacturer protocol Publication protocol 1 Relevant paper

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