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Found 1 matching solution for this experiment
|Seed cells with 150,000 cells/well|
| siRNA concentration:5–100 nmol/l
Higly effective siRNA: si-hVDAC1 1/A- S: -238-5′ACACUAGGCACCGAGAUUA 3′- 256, and AS: 5′UAAUCUCGGUGCCUAGUGU3′-256
Dilute siRNA and mix with jetPRIME® reagent.
Incubate for 10 to 15 min at RT and add to cells.
Incubate cells at 37 °C for 24 hours and change medium
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