siRNA / miRNA gene silencing Human - DU145 PACE4

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

PACE4 siRNA (h)

Santa Cruz Biotechnology

Genlantis

Upstream tips	Protocol tips	Downstream tips
	PACE4 siRNA concentration-100 nM Add incubated siRNA mixture (5 minutes at RT) and transfection mix (5 minutes at RT) to cells. Incubate at 37°C for 24 to 72 hours.	In case of Low Transfection Efficiency use buffer (100mM NaCl, 50mM Tris pH7.5 in RNase-free water) to dilute siRNA; water only denatures siRNA to improve the siRNA quality. In case of cell cytotoxicity, use less GeneSilencer(GS) Reagent and also lower the amount of siRNA used to keep GS:siRNA ratio optimized

Protocol tips
PACE4 siRNA concentration-100 nM Add incubated siRNA mixture (5 minutes at RT) and transfection mix (5 minutes at RT) to cells. Incubate at 37°C for 24 to 72 hours.
Downstream tips
In case of Low Transfection Efficiency use buffer (100mM NaCl, 50mM Tris pH7.5 in RNase-free water) to dilute siRNA; water only denatures siRNA to improve the siRNA quality. In case of cell cytotoxicity, use less GeneSilencer(GS) Reagent and also lower the amount of siRNA used to keep GS:siRNA ratio optimized

Manufacturer protocol Publication protocol 1 Relevant paper

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