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Thermo Fisher Scientific
|Seed 2.5 × 10^4 cells cm−2|
|siRNA sequence: 5′-GGCGCAGAUCGAUUUGAAU-3'
Final siRNA concentration of 1 μM.
Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).
Incubate for 5 minutes at room temperature and add to cells.
Replace medium after 24h and re-incubate cells for 1 day at 37°C.