siRNA / miRNA gene silencing Human - HaCaT VDAC1

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Start discussion

No discussions found

Share your thoughts or question with experts in your field

Start a discussion

Found 1 matching solution for this experiment

Accell Human VDAC1 (7416) siRNA - Set of 4

Dharmacon

JetPrime

Polyplus transfections

Upstream tips	Protocol tips	Downstream tips
Seed 150,000 cells/well	siRNA concentration- 5–100 nmol/l si-hVDAC1- S: 238-5′ACACUAGGCACCGAGAUUA3′-256 and AS: 5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 1/A- S: -238-5′ACACUAGGCACCGAGAUUA 3′- 256, and AS: 5′UAAUCUCGGUGCCUAGUGU3′-256; si-hVDAC1 2/A- S: 238-5′ ACACUAGGCACCGAGAUUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 2/B-, S: 238- 5′ACACUAGGCACCGAGA UUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′-256 Dilute siRNA and mix with jetPRIME® reagent. Incubate for 10 to 15 min at RT and add to cells. Incubate cells at 37 °C for 24 hours and change medium

Upstream tips
Seed 150,000 cells/well
Protocol tips
siRNA concentration- 5–100 nmol/l si-hVDAC1- S: 238-5′ACACUAGGCACCGAGAUUA3′-256 and AS: 5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 1/A- S: -238-5′ACACUAGGCACCGAGAUUA 3′- 256, and AS: 5′UAAUCUCGGUGCCUAGUGU3′-256; si-hVDAC1 2/A- S: 238-5′ ACACUAGGCACCGAGAUUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 2/B-, S: 238- 5′ACACUAGGCACCGAGA UUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′-256 Dilute siRNA and mix with jetPRIME® reagent. Incubate for 10 to 15 min at RT and add to cells. Incubate cells at 37 °C for 24 hours and change medium

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Fill out your contact details and receive price quotes in your Inbox