siRNA / miRNA gene silencing Human - HeLa SMU1

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Dharmacon

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-200 nM. Incubate the diluted oligonucleotide with diluted Oligofectamine mixture for 15-20 minutes at room temperature. Mix well and incubate the cells at 37°C in a CO2 incubator for 48 hours and add growth medium containing 3X the normal concentration of serum without removing the transfection mixture. Incubate cells for 48-72 h at 37°C in a CO2 incubator

Protocol tips
siRNA concentration-200 nM. Incubate the diluted oligonucleotide with diluted Oligofectamine mixture for 15-20 minutes at room temperature. Mix well and incubate the cells at 37°C in a CO2 incubator for 48 hours and add growth medium containing 3X the normal concentration of serum without removing the transfection mixture. Incubate cells for 48-72 h at 37°C in a CO2 incubator

Manufacturer protocol Publication protocol 1 Relevant paper

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