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Thermo Fisher Scientific
|siRNA concentration-200 nM.
Incubate the diluted oligonucleotide with diluted Oligofectamine mixture for 15-20 minutes at room temperature.
Mix well and incubate the cells at 37°C in a CO2 incubator for 48 hours and add growth medium containing 3X the normal concentration of serum without removing the transfection mixture.
Incubate cells for 48-72 h at 37°C in a CO2 incubator