siRNA / miRNA gene silencing Human - HT-1080 RELA

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Upstream tips
Pre-warm the supplemented Nucleofector Solution

pre-incubate plates filled with 1.0 ml of culture medium containing serum and supplements in a humidified 37°C/5% CO2 incubator
Protocol tips
seed 1x10^6 – 5x10^6 cells in the wells containing above medium and centrifuge at 90xg for 10 min.

Resuspend pellet and add Nucleofector Solution to a final concentration of 1x106 – 5x106
cells/100 μl

Mix 100 μl of cell suspension with siRNA
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