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Found 1 matching solution for this experiment
Silencer® GAPDH siRNA (human) + Lipofectamine® RNAiMAX Transfection Reagent - Delivery of siRNA
| Upstream tips |
Protocol tips |
Downstream tips |
| Seed 6.0 × 10^5 cells |
siRNA concentration-1–30 nM
Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).
Incubate for 5 minutes at room temperature and add to cells.
Incubate cells for 22h at 37°C |
|
| Upstream tips |
| Seed 6.0 × 10^5 cells |
| Protocol tips |
siRNA concentration-1–30 nM
Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).
Incubate for 5 minutes at room temperature and add to cells.
Incubate cells for 22h at 37°C |
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