siRNA / miRNA gene silencing Human - Jurkat GAPDH Lipid

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Found 1 matching solution for this experiment

Silencer® GAPDH siRNA (human) + Lipofectamine® RNAiMAX Transfection Reagent - Delivery of siRNA

Upstream tips
Seed 6.0 × 10^5 cells
Protocol tips
siRNA concentration-1–30 nM

Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).

Incubate for 5 minutes at room temperature and add to cells.

Incubate cells for 22h at 37°C
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