siRNA / miRNA gene silencing Human - LAMA84 AQP5

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Dharmacon

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-100 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).  Incubate for 5 minutes at room temperature and add to cells.  Replace the medium with RPMI culture medium supplemented with 10% FBS. Incubate cells further for analysis

Protocol tips
siRNA concentration-100 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).  Incubate for 5 minutes at room temperature and add to cells.  Replace the medium with RPMI culture medium supplemented with 10% FBS. Incubate cells further for analysis

Manufacturer protocol Publication protocol 1 Relevant paper

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