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|Seed 500,000 cells/ml.|
|Final concentration of 1 µM
Incubate TransIT-LT1 Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells.
Incubate for 48 hours and later plated in methylcellulose.
Assay cells after 72 h.