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| PACE4 siRNA concentration-100 nM
Add incubated siRNA mixture (5 minutes at RT) and transfection mix (5 minutes at RT) to cells.
Incubate at 37°C for 24 to 72 hours.
|In case of Low Transfection Efficiency use buffer (100mM NaCl, 50mM Tris pH7.5 in RNase-free water) to dilute siRNA; water only denatures siRNA to improve the siRNA quality.
In case of cell cytotoxicity, use less GeneSilencer(GS) Reagent and also lower the amount of siRNA used to keep GS:siRNA ratio optimized