siRNA / miRNA gene silencing Human - MDA-MB-231 bcl-2

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Bcl-2 siRNA (h)

Santa Cruz Biotechnology

Qiagen

Upstream tips	Protocol tips	Downstream tips
Seed 1.5 × 10^5/flask in 4 ml,	siRNA 5′ AACATCGCCCTGTGGATGACT-3′ Add diluted siRNA to HiPerFect Transfection Reagent to make a mixture   Incubate the mixture for 5–10 min at room temperature.  Add mixture to cells and incubate for 72 h

Upstream tips
Seed 1.5 × 10^5/flask in 4 ml,
Protocol tips
siRNA 5′ AACATCGCCCTGTGGATGACT-3′ Add diluted siRNA to HiPerFect Transfection Reagent to make a mixture   Incubate the mixture for 5–10 min at room temperature.  Add mixture to cells and incubate for 72 h

Manufacturer protocol Publication protocol 1 Relevant paper

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