siRNA / miRNA gene silencing Human - MDA-MB-231 DLX-2

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Dlx-2 siRNA (h)

Santa Cruz Biotechnology

HilyMax

Dojindo

Upstream tips	Protocol tips	Downstream tips
Seed 2.5×10^5 cells	siRNA concentartion-50 μM Add diluted siRNA to -HilyMax Transfection Reagent to make a mixture   Incubate the mixture for 15 min at room temperature.  Add mixture to cells and incubate for 24h

Upstream tips
Seed 2.5×10^5 cells
Protocol tips
siRNA concentartion-50 μM Add diluted siRNA to -HilyMax Transfection Reagent to make a mixture   Incubate the mixture for 15 min at room temperature.  Add mixture to cells and incubate for 24h

Manufacturer protocol Publication protocol 1 Relevant paper

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