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|Seed 500,000 cells/ml|
|Final concentration -1 µM
Incubate TransIT-LT1 Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells.
Incubate for 48 hours and plated in methylcellulose.
Re-incubate for another 72 h.