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|siRNA concentration-40 nM
Incubate the diluted oligonucleotide with diluted Oligofectamine mixture for 15-20 minutes at room temperature.
Mix well and incubate the cells at 37°C in a CO2 incubator for 4 hours and add growth medium containing 3X the normal concentration of serum without removing the transfection mixture.
Incubate cells for 24-72 h at 37°C in a CO2 incubator