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|Seed 1 × 10^5 cells per well|
|The DNMT1 siRNA sequences were 5′-GGAUGAGAAGAGACGUAGAtt-3′ and 5′-UCUACGUCUCUUCUCAUCCtg-3′, and the DNMT3B siRNA sequences were 5′-GCUCGUCUCCUAUCGAAAAtt-3′ and 5′-UUUUCGAUAGGAGACGAGCtt-3
Final concentrations - 7 nM
Add diluted siRNA to diluted Lipofectamine Reagent
Incubate for 5 min at RT and add to cells.
Replace medium after 6h and re-incubate cells for 2 days at 37°C.
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