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Found 1 matching solution for this experiment
Silencer® Select GLO-1 siRNA + Lipofectamine® 2000 Transfection Reagent
| Upstream tips |
Protocol tips |
Downstream tips |
| Seed 2 × 10^5 cells |
siRNA concentration-5 nM
Add diluted siRNA to diluted Lipofectamine Reagent
Incubate for 5 min at RT and add to cells.
Incubate cells for 1 day at 37°C and change medium.
Re-incbaute for another 48h |
|
| Upstream tips |
| Seed 2 × 10^5 cells |
| Protocol tips |
siRNA concentration-5 nM
Add diluted siRNA to diluted Lipofectamine Reagent
Incubate for 5 min at RT and add to cells.
Incubate cells for 1 day at 37°C and change medium.
Re-incbaute for another 48h |
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